![]() Extraction efficiency of formalin is 20–60 times more as compared to hand sorting, but hand sorting gives satisfactory results for earthworms of more than 0.2 g live weight, and cocoons can also be recovered by this method. This study explored the advantage and disadvantage of different sampling methods for the extraction of earthworms such as hand sorting, octet method, formalin method, mustard extraction method, allyl isothiocyanate (AITC), and onion extraction method. Different methods have been used for their extraction from the soil, and efficiency of each method is affected by physical properties, viz., temperature and moisture of the soil. Due to this property, their extraction from soil is tedious and time-consuming. Earthworms are so closely incorporated into the soil structure that they are not calculated easily from the soil.
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